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Addgene inc sp3 plasmid 24 541 expression vectors

Figure 4. GR and <t>Sp3</t> cooperatively transactivate the bICP0 E promoter. Neuro-2A cells were transfected with the designated EP-638 promoter constructs (0.5 µg DNA), plasmids that express GR and/or Sp3 proteins (1.0 µg DNA of each). Designated cultures were treated with 2% stripped FBS, and DEX (10 µM) was added to cultures. At 48 h after transfection, cells were harvested and protein lysate subjected to dual-luciferase assay. Promoter activity levels in the sample containing WT EP-638 co-transfected with only an empty vector and only treated with DMSO (no hormone) were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to respective construct alone (*, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001). A hash (#) denotes significant differences between indicated groups (#, p < 0.05 and ##, p < 0.01) in the transactivation of wt EP-638 co-transfected with GR and Sp3 relative to Neuro-2A cells transfected with mutant EP-638 constructs.

Sp3 Plasmid 24 541 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 13 article reviews

sp3 plasmid 24 541 expression vectors - by Bioz Stars, 2026-06

93/100 stars

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1) Product Images from "Glucocorticoid Receptor (GR) and Specificity Protein 1 (Sp1) or Sp3 Transactivate the Bovine Alphaherpesvirus 1 (BoHV-1)-Infected Cell Protein 0 Early Promoter."

Article Title: Glucocorticoid Receptor (GR) and Specificity Protein 1 (Sp1) or Sp3 Transactivate the Bovine Alphaherpesvirus 1 (BoHV-1)-Infected Cell Protein 0 Early Promoter.

Journal: Viruses

doi: 10.3390/v17020229

Figure 4. GR and Sp3 cooperatively transactivate the bICP0 E promoter. Neuro-2A cells were transfected with the designated EP-638 promoter constructs (0.5 µg DNA), plasmids that express GR and/or Sp3 proteins (1.0 µg DNA of each). Designated cultures were treated with 2% stripped FBS, and DEX (10 µM) was added to cultures. At 48 h after transfection, cells were harvested and protein lysate subjected to dual-luciferase assay. Promoter activity levels in the sample containing WT EP-638 co-transfected with only an empty vector and only treated with DMSO (no hormone) were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to respective construct alone (*, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001). A hash (#) denotes significant differences between indicated groups (#, p < 0.05 and ##, p < 0.01) in the transactivation of wt EP-638 co-transfected with GR and Sp3 relative to Neuro-2A cells transfected with mutant EP-638 constructs.

Figure Legend Snippet: Figure 4. GR and Sp3 cooperatively transactivate the bICP0 E promoter. Neuro-2A cells were transfected with the designated EP-638 promoter constructs (0.5 µg DNA), plasmids that express GR and/or Sp3 proteins (1.0 µg DNA of each). Designated cultures were treated with 2% stripped FBS, and DEX (10 µM) was added to cultures. At 48 h after transfection, cells were harvested and protein lysate subjected to dual-luciferase assay. Promoter activity levels in the sample containing WT EP-638 co-transfected with only an empty vector and only treated with DMSO (no hormone) were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to respective construct alone (*, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001). A hash (#) denotes significant differences between indicated groups (#, p < 0.05 and ##, p < 0.01) in the transactivation of wt EP-638 co-transfected with GR and Sp3 relative to Neuro-2A cells transfected with mutant EP-638 constructs.

Techniques Used: Transfection, Construct, Luciferase, Activity Assay, Plasmid Preparation, Activation Assay, Mutagenesis

Figure 5. Sp3 increased the KLF4-mediated transactivation of the wt bICP0 E promoter. Neuro-2A cells were transfected with the designated EP-638 promoter constructs (0.5 µg DNA), KLF4 and/or Sp3 (1 µg DNA of each). The designated cultures were treated with 2% stripped FBS, and DEX (10 µM) was added to cultures. At 48 h after transfection, cells were harvested and protein lysate subjected to dual-luciferase assay. Promoter activity levels in the sample containing WT EP-638 co-transfected with only an empty vector and only treated with DMSO (no hormone) were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05; and ****, p < 0.0001). A hash denotes significant differences between indicated groups (#, p < 0.05 and ##, p < 0.01) in the transactivation of wt EP-638, co-transfected with a KLF4 and Sp3 relative to Neuro-2A cells transfected with mutant EP-638 constructs.

Figure Legend Snippet: Figure 5. Sp3 increased the KLF4-mediated transactivation of the wt bICP0 E promoter. Neuro-2A cells were transfected with the designated EP-638 promoter constructs (0.5 µg DNA), KLF4 and/or Sp3 (1 µg DNA of each). The designated cultures were treated with 2% stripped FBS, and DEX (10 µM) was added to cultures. At 48 h after transfection, cells were harvested and protein lysate subjected to dual-luciferase assay. Promoter activity levels in the sample containing WT EP-638 co-transfected with only an empty vector and only treated with DMSO (no hormone) were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05; and ****, p < 0.0001). A hash denotes significant differences between indicated groups (#, p < 0.05 and ##, p < 0.01) in the transactivation of wt EP-638, co-transfected with a KLF4 and Sp3 relative to Neuro-2A cells transfected with mutant EP-638 constructs.

Techniques Used: Transfection, Construct, Luciferase, Activity Assay, Plasmid Preparation, Activation Assay, Mutagenesis

Figure 6. Analysis of cooperative interactions between GR and Sp3 or KLF4 and Sp3 of EP-638 promoter activity. (A) Neuro-2A cells were transfected with the EP-638 promoter construct (0.5 µg DNA), GR (1 µg DNA) and increasing concentrations of Sp3, as denoted. (B) Neuro-2A cells were transfected with the EP-638 promoter construct (0.5 µg DNA), KLF4 (1 µg DNA), and increasing concentrations of Sp3, as denoted. Promoter activity levels in the sample containing wt EP-638 co-transfected with only an empty vector were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001) or transactivation of wt EP-638 co-transfected with GR and Sp3 in Neuro-2A cells.

Figure Legend Snippet: Figure 6. Analysis of cooperative interactions between GR and Sp3 or KLF4 and Sp3 of EP-638 promoter activity. (A) Neuro-2A cells were transfected with the EP-638 promoter construct (0.5 µg DNA), GR (1 µg DNA) and increasing concentrations of Sp3, as denoted. (B) Neuro-2A cells were transfected with the EP-638 promoter construct (0.5 µg DNA), KLF4 (1 µg DNA), and increasing concentrations of Sp3, as denoted. Promoter activity levels in the sample containing wt EP-638 co-transfected with only an empty vector were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001) or transactivation of wt EP-638 co-transfected with GR and Sp3 in Neuro-2A cells.

Techniques Used: Activity Assay, Transfection, Construct, Plasmid Preparation, Activation Assay

Figure 7. GR, Sp3, and/or KLF4 stimulate productive infection. (A) Neuro-2A cells were transfected with 1.0 µg BoHV-1 gCblue DNA and, where indicated, a plasmid expressing mouse GR protein (1.0 µg), Sp3 (1.0 µg), and/or KLF4 (1.0 µg). To maintain the same amount of DNA in each sample, an empty vector was included in the samples. After transfection, 2% stripped FBS was added to the medium. Designated cultures were then treated with water-soluble DEX (10 µM; Sigma) 24 h post- transfection. At 48 h after transfection, the number of β-Gal+ cells were counted. The value for the control (gCblue virus co-transfected with empty vector and then treated with PBS after transfection) was set to 1. (B) The results from DEX-treated cultures were compared to those from the control. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05).

Figure Legend Snippet: Figure 7. GR, Sp3, and/or KLF4 stimulate productive infection. (A) Neuro-2A cells were transfected with 1.0 µg BoHV-1 gCblue DNA and, where indicated, a plasmid expressing mouse GR protein (1.0 µg), Sp3 (1.0 µg), and/or KLF4 (1.0 µg). To maintain the same amount of DNA in each sample, an empty vector was included in the samples. After transfection, 2% stripped FBS was added to the medium. Designated cultures were then treated with water-soluble DEX (10 µM; Sigma) 24 h post- transfection. At 48 h after transfection, the number of β-Gal+ cells were counted. The value for the control (gCblue virus co-transfected with empty vector and then treated with PBS after transfection) was set to 1. (B) The results from DEX-treated cultures were compared to those from the control. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05).

Techniques Used: Infection, Transfection, Plasmid Preparation, Expressing, Control, Virus, Construct

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Journal: Viruses

Article Title: Glucocorticoid Receptor (GR) and Specificity Protein 1 (Sp1) or Sp3 Transactivate the Bovine Alphaherpesvirus 1 (BoHV-1)-Infected Cell Protein 0 Early Promoter.

doi: 10.3390/v17020229

Figure Lengend Snippet: Figure 4. GR and Sp3 cooperatively transactivate the bICP0 E promoter. Neuro-2A cells were transfected with the designated EP-638 promoter constructs (0.5 µg DNA), plasmids that express GR and/or Sp3 proteins (1.0 µg DNA of each). Designated cultures were treated with 2% stripped FBS, and DEX (10 µM) was added to cultures. At 48 h after transfection, cells were harvested and protein lysate subjected to dual-luciferase assay. Promoter activity levels in the sample containing WT EP-638 co-transfected with only an empty vector and only treated with DMSO (no hormone) were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to respective construct alone (*, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001). A hash (#) denotes significant differences between indicated groups (#, p < 0.05 and ##, p < 0.01) in the transactivation of wt EP-638 co-transfected with GR and Sp3 relative to Neuro-2A cells transfected with mutant EP-638 constructs.

Article Snippet: The Sp1 (plasmid #27,264) and Sp3 (plasmid #24,541) expression vectors were purchased from Addgene.

Techniques: Transfection, Construct, Luciferase, Activity Assay, Plasmid Preparation, Activation Assay, Mutagenesis

Journal: Viruses

Article Title: Glucocorticoid Receptor (GR) and Specificity Protein 1 (Sp1) or Sp3 Transactivate the Bovine Alphaherpesvirus 1 (BoHV-1)-Infected Cell Protein 0 Early Promoter.

doi: 10.3390/v17020229

Figure Lengend Snippet: Figure 5. Sp3 increased the KLF4-mediated transactivation of the wt bICP0 E promoter. Neuro-2A cells were transfected with the designated EP-638 promoter constructs (0.5 µg DNA), KLF4 and/or Sp3 (1 µg DNA of each). The designated cultures were treated with 2% stripped FBS, and DEX (10 µM) was added to cultures. At 48 h after transfection, cells were harvested and protein lysate subjected to dual-luciferase assay. Promoter activity levels in the sample containing WT EP-638 co-transfected with only an empty vector and only treated with DMSO (no hormone) were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05; and ****, p < 0.0001). A hash denotes significant differences between indicated groups (#, p < 0.05 and ##, p < 0.01) in the transactivation of wt EP-638, co-transfected with a KLF4 and Sp3 relative to Neuro-2A cells transfected with mutant EP-638 constructs.

Article Snippet: The Sp1 (plasmid #27,264) and Sp3 (plasmid #24,541) expression vectors were purchased from Addgene.

Techniques: Transfection, Construct, Luciferase, Activity Assay, Plasmid Preparation, Activation Assay, Mutagenesis

Journal: Viruses

Article Title: Glucocorticoid Receptor (GR) and Specificity Protein 1 (Sp1) or Sp3 Transactivate the Bovine Alphaherpesvirus 1 (BoHV-1)-Infected Cell Protein 0 Early Promoter.

doi: 10.3390/v17020229

Figure Lengend Snippet: Figure 6. Analysis of cooperative interactions between GR and Sp3 or KLF4 and Sp3 of EP-638 promoter activity. (A) Neuro-2A cells were transfected with the EP-638 promoter construct (0.5 µg DNA), GR (1 µg DNA) and increasing concentrations of Sp3, as denoted. (B) Neuro-2A cells were transfected with the EP-638 promoter construct (0.5 µg DNA), KLF4 (1 µg DNA), and increasing concentrations of Sp3, as denoted. Promoter activity levels in the sample containing wt EP-638 co-transfected with only an empty vector were normalized to a value of 1, with fold activation for other samples presented. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05; **, p < 0.01; ***, p < 0.001; and ****, p < 0.0001) or transactivation of wt EP-638 co-transfected with GR and Sp3 in Neuro-2A cells.

Article Snippet: The Sp1 (plasmid #27,264) and Sp3 (plasmid #24,541) expression vectors were purchased from Addgene.

Techniques: Activity Assay, Transfection, Construct, Plasmid Preparation, Activation Assay

Journal: Viruses

Article Title: Glucocorticoid Receptor (GR) and Specificity Protein 1 (Sp1) or Sp3 Transactivate the Bovine Alphaherpesvirus 1 (BoHV-1)-Infected Cell Protein 0 Early Promoter.

doi: 10.3390/v17020229

Figure Lengend Snippet: Figure 7. GR, Sp3, and/or KLF4 stimulate productive infection. (A) Neuro-2A cells were transfected with 1.0 µg BoHV-1 gCblue DNA and, where indicated, a plasmid expressing mouse GR protein (1.0 µg), Sp3 (1.0 µg), and/or KLF4 (1.0 µg). To maintain the same amount of DNA in each sample, an empty vector was included in the samples. After transfection, 2% stripped FBS was added to the medium. Designated cultures were then treated with water-soluble DEX (10 µM; Sigma) 24 h post- transfection. At 48 h after transfection, the number of β-Gal+ cells were counted. The value for the control (gCblue virus co-transfected with empty vector and then treated with PBS after transfection) was set to 1. (B) The results from DEX-treated cultures were compared to those from the control. The results are the average of 3 independent experiments and error bars denote the standard error. Two-way ANOVA test was used for analyzing the results. An asterisk denotes significant differences compared to the respective construct alone (*, p < 0.05).

Article Snippet: The Sp1 (plasmid #27,264) and Sp3 (plasmid #24,541) expression vectors were purchased from Addgene.

Techniques: Infection, Transfection, Plasmid Preparation, Expressing, Control, Virus, Construct

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1) Product Images from "Glucocorticoid Receptor (GR) and Specificity Protein 1 (Sp1) or Sp3 Transactivate the Bovine Alphaherpesvirus 1 (BoHV-1)-Infected Cell Protein 0 Early Promoter."

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